Amino columns are most commonly used in normal phase HPLC mode however, the Cogent NP™ Amino column can also be used in reversed phase or HILIC mode if needed. The amino functionality of the amino-propyl ligand has a lone electron pair. Therefore, the hydrocarbon chain (C3) will be polar and will have a slightly negative dipole moment toward the Nitrogen of the amino group making the stationary phase polar.
Different molecules will have different retention based on their polarity and size (access to the ligand) thus, producing a unique selectivity.