Capillary and Nano Columns
- Capillary (500μm and 300μm) and nano (100μm and 75μm) dimensions
- Wide range of bonded phases available
- 100Å and 300Å pore sizes
- High efficiency, long lifetime and guaranteed reproducibility
- LC-MS and LC-MS/MS applications
ACE Ranges
ACE Capillary & Nano
In addition to the extensive range of analytical (1.0-4.6mm id) through to preparative (21.2-50mm id) columns, ACE columns are now available in capillary (500μm and 300μm) and nano (100μm and 75μm) dimensions.ACE capillary and nano columns are available with all ACE bonded phase chemistries in both 100Å and 300Å pore sizes. The same features that make ACE ultra-inert base deactivated columns the choice of method development chemists also make them the ideal choice for capillary and nano HPLC applications.
Improved Mass Limit of Detection
Capillary and nano HPLC is gaining acceptance for applications where limited sample amounts lead to problems in detection sensitivity. This is relevant in the areas of pharmacokinetics, trace analysis and in particular the expanding fields of bioanalytical and proteomic analysis.ACE capillary and nano columns are ideal for use with detectors requiring very low flow rates, such as electrospray LC-MS.
ACE capillary and nano HPLC columns offer high sensitivity due to their low dispersion characteristics. The table below shows the theoretical sensitivity increase of each id column compared with a 4.6mm id analytical column and 1mm id microbore column. This increase in sensitivity can be important for accurate quantitation of sample limited applications. For maximum performance, columns should be used with fully optimized HPLC systems (eg. minimize system dead volume using short lengths of <75μm connection tubing).
Table 1: Sensitivity increase
| Column id (mm) | Typical flow rate (µl/min) | Theoretical sensitivity increase* |
|---|---|---|
| 4.6 | 1000 | 1 |
| 1.0 | 40 | 21 |
| 0.5 | 10 | 85 |
| 0.3 | 3 | 235 |
| 0.1 | 0.5 | 2100 |
| 0.075 | 0.3 | 3760 |
ACE capillary and nano columns are available with all bonded phase chemistries, 100Å or 300Å pore sizes and 3, 5 or 10μm particle sizes.
Trace Enrichment/Guard Columns
Capillary HPLC guard columns (5mm x 300μm or 500μm id) prolong the lifetime of the capillary column. They are also suitable for trace enrichment and column switching applications, particularly for concentration of low abundance analytes or desalting of biological samples. These short columns can be used to separate analyte from matrix" prior to analysis with detectors such as ESI-MS, where baseline resolution is not required.