Obelisc

OBELISC

Two complimentary columns, Obelisc R and Obelisc N, based on Liquid Separation Cell ( LiSC ™) technology, offer a new approach to separate a variety of small molecules. Buffer concentration, buffer pH, and organic modifier concentration are three orthogonal parameters of the mobile phase that allow adjustment of column properties to separate complex mixtures.

OBELISC R

Obelisc R is a mixed-mode analytical column designed for use in traditional reversedphase type applications.

• – Thanks to the presence of ionic groups and a long hydrophobic chain, Obelisc R provides additional retention and tuning that traditional reversedphase columns cannot offer.
• – Multiple separation modes available (RP, NP, IE)
• – Compatible with LC-MS, ELSD, preparative chromatography, and low UV (<220 nm); works with low concentration buffers.

Column Diameter	0.5 mm, 1 mm, 2.1 mm, 3.2 mm, 4.6 mm, 10 mm, 22 mm
Column Length	10 mm, 25 mm, 50 mm, 100 mm, 150 mm, 250 mm, Guard
Particle Size	5 µm, 10 µm
Pore Size	100 A

OBELISC N

Obelisc N is a mixed-mode column characterized by its highly polar properties, making it well-suited for separating polar and charged analytes.

• Obelisc N features both positively and negatively charged groups, allowing it to interact with analytes that are either positively or negatively charged.
• – The column supports multiple separation modes, including normal phase (NP), hydrophilic interaction liquid chromatography (HILIC), and ion exclusion (IE).
• – In HILIC mode, Obelisc N displays different selectivity compared to other HILIC or bare silica columns.
• – The column is compatible with liquid chromatography-mass spectrometry (LC-MS), evaporative light scattering detection (ELSD), preparative chromatography, and low ultraviolet (UV) detection below 220 nm, using low concentration buffers.

Column Diameter	0.5 mm, 1 mm, 2.1 mm, 3.2 mm, 4.6 mm, 10 mm, 22 mm
Column Length	10 mm, 25 mm, 50 mm, 100 mm, 150 mm, 250 mm, Guard
Particle Size	5 µm
Pore Size	100 A