ACE® AQ columns provide great reproducibility and column lifetime. These stainless steel columns are available in a wide range of particle sizes and dimensions from capillary to preparative.
When separating very polar, water-soluble compounds, highly aqueous (>95%) mobile phases are often required to achieve sufficient retention. However, operating a conventional C18 column under such conditions can lead to poor chromatographic reproducibility. Over time peaks will elute with shorter and shorter retention times and resolution between peaks will deteriorate. ACE AQ columns introduce an integral polar functionality, which prevents this retention loss when using highly aqueous mobile phases.
ACE AQ is an ultra-inert phase designed for the reversed-phase separation of very polar compounds with up to 100% aqueous mobile phase. ACE AQ is ideal for use with fast gradients due to its rapid re-equilibration properties and resistance to retention loss.
| Phase | Functional Group | Endcapped | Particle size (µm) | Pore size (Å) | Surface area (m2/g) | Carbon load (%) |
|---|---|---|---|---|---|---|
| AQ | Proprietary | yes | 3,5,10 | 100 | 300 | 14.0 |
Many columns designed for use under high aqueous conditions are compromised by the low purity silica employed and exhibit poor peak shape with polar basic molecules. This results in poor chromatography and ultimately leads to poor column reproducibility. ACE AQ columns are manufactured from the same ultrainert, high purity silica as all ACE phases – ensuring excellent chromatography and excellent reproducibility are obtained.
