ACE -C18-Amide

ACE C18-Amide

For increased polar retention and alternative selectivity

ACE® C18-Amide is a uniquely designed stationary phase offering improved retention of polar compounds, enhanced phenolic resolution and alternative selectivity to C18 bonded columns. These stainless steel columns are available in a wide range of particle sizes and dimensions from capillary to preparative.

  • Ultra-high purity, base deactivated silica with exceptional reproducibility
  • Ideal for a wide range of analytes differing in hydrophobicity
  • Wide range of particle sizes (2.0 to 10 µm) and complementary phases
  • Low bleed for UV and LC-MS compatibility
  • C18 hydrophobic plus Amide interactions, compatible with 100% aqueous conditions
  • Resolution
  • Alternative Selectivity
  • Applications
  • Enhance selectivity

Leverage the Power of Selectivity with ACE C18-Amide:

The resolution equation determines the parameters that contribute to resolution; N, α and k. In recent years there has been a significant focus on the use of ultra efficient “UHPLC” columns (such as ACE Excel 2μm columns) as a means of achieving separation goals.

However, selectivity is often overlooked. This is unfortunate as of the three parameters that affect resolution, selectivity is the most powerful. By leveraging both efficiency and selectivity, better and faster separations can often be achieved.

How does ACE C18-Amide provide Alternative Selectivity?

  • ACE C18-Amide combines a C18 with a polar amide group on a single ligand
  • Extended spacer technology additionally provides extended column lifetime
    • Separation of neutral compounds under RP condition

Recommended Applications:

  • Small water soluble analytes and polar compounds
  • H-bond donors, acids, bases and phenolic compounds
  • Small peptides

This application illustrates the difference between two ACE bonded phases, C18-Amide and C18. Although both phases offer the possibility of strong hydrophobic interaction from their respective C18 chains, the amide group embedded within the C18-Amide phase introduces additional modes of interaction which ultimately increases retention for polar compounds and provides alternative selectivity.