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ACE -C18-Amide

ACE -C18-Amide

For increased polar retention and alternative selectivity

ACE C18-Amide is the latest addition to the ACE range of bonded phases which offer both complementary selectivity & the option of 2μm, 3μm, 5μm and 10μm particle sizes - to enable development of rugged, robust UHPLC & HPLC methods.

  • Alternative selectivity for method development
  • Improved separations with polar, acidic, basic and phenolic compounds
  • High efficiency 2μm, 3μm, 5μm and 10μm particles for UHPLC and HPLC
  • Ultra-inert for maximum performance and reproducibility
  • Resolution
  • Alternative Selectivity
  • Applications
  • Enhance selectivity

Leverage the Power of Selectivity with ACE C18-Amide:

The resolution equation determines the parameters that contribute to resolution; N, α and k. In recent years there has been a significant focus on the use of ultra efficient “UHPLC” columns (such as ACE Excel 2μm columns) as a means of achieving separation goals.

However, selectivity is often overlooked. This is unfortunate as of the three parameters that affect resolution, selectivity is the most powerful. By leveraging both efficiency and selectivity, better and faster separations can often be achieved.

How does ACE C18-Amide provide Alternative Selectivity?

  • ACE C18-Amide combines a C18 with a polar amide group on a single ligand
  • Extended spacer technology additionally provides extended column lifetime
    • Separation of neutral compounds under RP condition

Recommended Applications:

  • Small water soluble analytes and polar compounds
  • H-bond donors, acids, bases and phenolic compounds
  • Small peptides

This application illustrates the difference between two ACE bonded phases, C18-Amide and C18. Although both phases offer the possibility of strong hydrophobic interaction from their respective C18 chains, the amide group embedded within the C18-Amide phase introduces additional modes of interaction which ultimately increases retention for polar compounds and provides alternative selectivity.